Meaning
Statistical validation method used in analytical chemistry to determine the accuracy of a measurement by adding a known amount of analyte to a sample. Calculating the spiked recovery involves comparing the measured value of the enriched sample against the expected total. High percentages indicate that the testing method and equipment are performing correctly without loss of the target substance.
Addition Accuracy
The precision of the initial spike determines the reliability of the entire validation exercise. Technicians must use calibrated pipettes or microbalances to introduce a specific volume of the target chemical into the polymer matrix. Any error at this stage will produce an incorrect recovery percentage that might falsely suggest a problem with the analytical equipment.
Method Verification
Successful recovery of the added material proves that the extraction and detection steps are functioning as intended. If the measured value is much lower than the spike, it suggests that the analyte is being trapped by the polymer or lost during the heating phase. This check is a requirement for laboratories seeking accreditation for environmental or safety testing of plastic parts.
Matrix Influence
Different polymer types can interfere with the detection of additives through chemical or physical shielding. A spiked recovery test reveals how the presence of pigments or fillers affects the ability of the sensor to see the target molecule. Adjusting the preparation method based on these results ensures that the final reported concentration is accurate regardless of the background material.
